Yes... your frame can have multiple panels as its children, and each panel can independently have layout managed by separate types of managers. what follows is just a crude example...

//somewhere in the frame's layout code, where
//the frame has borderlayout running...

Panel p1 = new Panel();
p1.setLayout(new FlowLayout());
p1.add(new JLabel("Foo"));
p2.add(new JLabel("Bar"));

Panel p2 = new Panel();
p2.setLayout(new GridLayout(1,2));
p2.add(new JLabel("One");
p2.add(new JLabel("Two");

this.add(p1, "Center");
this.add(p2, "South");

// etc

IIRC, any Container can have a separate layout manager. Since Panel is a subclass of Container, each Panel can have it's own layout manager.

Learn to use the API docs.